Protein nokuguqulwa

Ukumila (isakhiwo) chain peptide lubekwa kwaye iyodwa kwizinongo nganye. Xa iimeko ezizodwa kukho umsantsa inani elikhulu neentambo leyo uzinzise isakhiwo komhlaba kwemolekyuli compound. Ngenxa yoko, umsantsa ngokupheleleyo yonke molecule (okanye inxalenye enkulu yayo) kuthatha uhlobo coil random. Le nkqubo ibizwa ngokuba "nokuguqulwa". Olu tshintsho zingabangela ubushushu ukusuka degrees ezingamashumi amathandathu ukuya asibhozo. Ngenxa yoko, molecule nganye, umsantsa nto isenokwahluka Ukumila evela kwenye.

Nokuguqulwa kweziyi kwenzeka phantsi kwempembelelo naziphi na izinto ekwaziyo kutshabalalisa iibhondi non-covalent. Le nkqubo kwenzeka phantsi kweemeko obrak okanye emuncu, phezu komhlaba icandelo nqanaba, phantsi intshukumo ezithile khompawundi eziphilayo (phenols, alkoholi kunye nabanye). protein nokuguqulwa kunokwenzeka phantsi kwempembelelo guanidinium chloride okanye urea. lathi ifomu iibhondi ezibuthathaka (hydrophobic, ionic, hydrogen) kunye carbonyl okanye acid amaqela kwezibonelelo peptide kunye nenani amaqela zeentsalela acid acid, endaweni yabo ezikhoyo protein imixokelelwane hydrogen ngaphakathi iimolekyuli. Ngenxa yoko, utshintsho lwenzeka kubume lesibini nelesithathu.

Ukumelana kwiiarhente denaturing ixhomekeka isikakhulu kwindlela ubukho kwi molecule imichiza nezo iintambo disulfide. Trypsin inhibitor ibe iibhondi S ezintathu - S. Phantsi imeko kwakhona protein nokuguqulwa lwenzeka ngaphandle nezinye iimpembelelo. Ukuba emva koko abeke uqhagamshelwano kwiimeko apho igcwala na SH-amaqela cysteine kunye ukuyilwa iibhondi disulfide ngaphandle, Ukumila abuyiselwe. Ukubakho nkqu iqhina disulfide olunye kuphula uzinzo isakhiwo lwendawo.

protein nokuguqulwa, ngokokuqhelekileyo sikhatshwe ekuncipheni ukunyibilika. Kunye nale kwenze rhoqo iifom. Oku kwenzeka ngohlobo "protini curdled." Xa lamanani eliphezulu le khompawundi kwi isisombululo, "coagulation" yeyona isisombululo lonke, njengoko, umzekelo, oku kwenzeka xa abilayo amaqanda. Xa nokuguqulwa ye kwiproteni ulahlekelwa umsebenzi wayo omzalayo. Lo mgaqo isekelwe ukusetyenziswa carbolic acid (phenol zolwelo isisombululo) njenge into kubola.

Xenga lobume lwendawo, amathuba eliphezulu ukusilela phantsi kwempembelelo arhente ezahlukeneyo kunzima kakhulu ukuba ikheswa nophando protein. Kwakhona wadala iingxaki ezithile xa usebenzisa khompawundi amayeza kunye noshishino.

Ukuba nokuguqulwa protein lwaqhutywa yi ukubekwa kumaqondo obushushu aphezulu, ukupholisa olucothayo kwenzeka phantsi kweemeko ezithile inkqubo renativatsii - kwakhona nakozalelwe Ukumila (original). Oku kubonisa ukuba kungekabekwa chain peptide luhambiselane ne isakhiwo ephambili.

Lokusekwa Ukumila zomthonyama (umthombo indawo) yinkqubo ezikhululekileyo. Ngamanye amazwi, eli lungiselelo kulingana isixa ubuncinane amandla ezamahala eziqulethwe molecule. Umntu ngenxa yoko kugqitywe ukuba ubume bendawo kwi compound ufakwe kwi ukulandelana acid acid kwi imixokelelwane peptide. Oku kuthetha ukuba zonke polypeptides ezinxulumene yi alternation ye asidi acid (umzekelo, amatyathanga mioglobinovye peptide), uya kuthatha i Ukumila efanayo.

Iiproteni ingaba umahluko ezibalulekileyo kubume zaseprayimari, nokuba incinane, nokuba twatse ngokugqithisileyo Ukumila.