Vitrification of oocytes: ntoni na?

Kwiminyaka yakutshanje, in vitro liphuhlisa ngokukhawuleza kakhulu. Le nkqubo ayisa uthathwa ukuba babe yinto eningenakukholwa kwaye ivalwe bangayisebenzisi ngabantu abaqhelekileyo. IVF lwenziwa kulo lonke ihlabathi. iilebhu ezizodwa kunye neeklinikhi ziyafumaneka kwimizi ngemizi enkulu. Ngonyaka ukuphuhlisa, ukuphuculwa kweendlela ezintsha okuzala. Ngokusebenzisa uphuhliso yale ndlela, izibini ezininzi ongachumiyo bakwazi ukuba babe abazali. Ngokufuthi nokuqokelelwa kolwazi malunga IVF, abasetyhini bajongene ibinzana ezifana vitrification of oocytes. Eli gama libhekisela inkqubo entsha noko ekuvumela ukuba ukuphucula umgangatho amaqanda.

Le mbono "kweqanda vitrification" - yintoni na?

Ukuze siqonde indlela inkqubo vitrification, kufuneka ube ufifi neqanda. Le nkqubo ibandakanya isampuli kweeseli lwegciwane obhinqileyo (oocytes) ukusuka zamaqanda. Ngoko kukho abo ukucocwa neqanda onesifo okanye iqabane (ukuba indoda ayikwazi ukuba abantwana). Emva koku, le elilodwa share ibekwa em- zesibeleko. Vitrification of oocytes - inkqubo efanayo ukuya cryopreservation of oocytes. Noko ke, oko phambili ngakumbi. Phantsi vitrification kwakuthetha ukuqanda of akuqolanga iiseli lwegciwane. Ngenxa yoko, le oocytes nga ugcinwe ixesha elide. Nangona urhulumente "engumkhenkce", iiseli Vitrified iqhubeka iphila. Le nkqubo kuthathwa ukuba idabi enkulu amayeza, kuba ebulela abafazi banako ukuba usana, nkqu yokungabikho zokuzala.

Yintoni injongo kweqanda vitrification lwenziwa?

EYurophu, ingqiqo ezifana cryopreservation, vitrification imibungu kunye oocytes, ziye lwaziwa ixesha elide. Oku kunokubonwa kwi yentshona umboniso phezu iyeza. Xa amazwe awayesakuba yiSoviet Union, kakhulu, oogqirha eziqhelekileyo kunye la magama eminyaka. Nangona kunjalo, ezi nkqubo ziye zifumaneke kwizigulane kutshanje. Kwiimeko ezininzi, vitrification of oocytes zaveliswa ngenxa neqanda. Ngexesha qhwa iiseli ubuntwana ngendlela Umfazi xa athe banethuba elibhetele IVF ngempumelelo. Nebhetshi yokuqala yamaphepha oocytes ezikhethwe isizalo akakwazi ukuhlala phantsi. Kulo kwakhona isampuli zokufunda asifuneki, njengoko kukho iiseli esele Vitrified. Ukongeza, kukho iimeko xa isibini engenazintsholongwane acele nokulondolozwa oocytes ixesha elide. oko kudla kufuneka kwiimeko ezahlukeneyo zophendlo lwezifo apho umfazi akakwazi abe nabantwana. Kwakhona, kunye sicelo azithumela nini na izigulane ekulungiseleleni utyando ukususa amalungu okuzala.

Makungaze naphantsi kwazo ezimbi isindululo amaqanda vitrification?

Vitrification of oocytes Amadlozi - inqubo paid electricity meters wonke. eziveza Special kuba akanayo, nangona kunjalo, ukuba kuyimfuneko kuphela kwiimeko ezithile. Oku akuthethi ukuba abanye abafazi nako vitrified iiseli zabo. Noko ke, xa kungekho izizathu qhwa of oocytes ngendlela efanayo kwizigulane bacebisa cryopreservation. Amaninzi vitrification lwenziwa phantsi kwale miqathango ilandelayo:

1. Kulungiselelwa ovariectomy. Lo msebenzi wenziwa umhlaza ovarian kunye bangathenjwa yophuhliso lwayo. Esi sizathu ayikho yande kakhulu, ekubeni kwiimeko ezininzi, ezimbi umhlaza yamanina ishenxiswe kunye wesibeleko.
2. Ukungabi nako ukufumana amadlozi inani imihla oocytes. Ngenxa yesi sizathu, iiseli kufuneka engumkhenkce nje ukuchumisa umbandela akuyi kunikwa.
3. Umhlaza nayiphi lizwe. Kungakhathaliseki apho iye nomhlaza kufuna imichiza iziyobisi radiotherapy kunye nonyango (kweeseli). Nganye kwezi ndlela ezingafunekiyo iziphumo zokukhulelwa. Emva kwezi unyango kutshabalalisa umhlaza nje kuphela, kodwa iiseli ezinempilo, kuquka oocytes.
4. umnqweno bhinqa ukuba abantwana kwixesha ekude, umzekelo, emva ukuya exesheni. Kwiimeko ezinjalo kunqabile cryopreservation ezihle kuba iqanda amazinyo ngale ndlela iphila ixesha elide. Ngoko ke, oogqirha bathi enye indlela - vitrification of oocytes.
5. Ukuphinda IVF, ukuba kwixesha lokuqala kwamaqanda babengaqhelananga. Kulo mzekelo, umnikelo vitrification ukuze sithathe izinto kathathu.

Vitrified oocytes donor - ntoni na?

Kwezinye iimeko, umntu umphunga engenazintsholongwane akakwazi ukukhawula umntwana, nkqu ngoncedo neqanda. Sithetha ngayo amabhinqa umsebenzi othile le gonads. Ukulungiselela le njongo, iibhanki abanikeli oocytes zaphuhliswa. Ngelishwa, bona bengekho kuwo onke amazwe. Zonke iiseli lwegciwane angaqolanga na urhulumente vitrified, ukuze ukuba igcinwe. Ubungqina obubonakalayo buxela ukuba IVF usebenzisa oocytes anikelayo zezi:

1. ngezifo zemfuza uzimisele - ovarian ukusilela syndrome.
2. Ukungabikho oocytes ngenxa iimpawu ubudala uhlelo zokuzala (engama-40 nangaphezulu).
3. Ukungabi nako ukuvuselela ovulation ngenxa nongenelelo zotyando kwi zamaqanda, ichemotherapy sesichengeni.
4. Ingozi yokuba umntwana sisifo yemfuza.
5. Ukuphinda IVF, siye na iziphumo emihle ngenxa yokuba "mgangathweni" amaqanda abo.

Ngubani na ube oocytes anikelayo vitrified: Iimfuno

Ucwangciso-ntsapho - oku sisigqibo esinzulu kakhulu, ingakumbi kwiimeko xa isibini onyanzelwayo ukuba ukusebenzisa iiseli mali. Ngaphambi kokuba ufumane oocytes baze ukuba vitrification, ugqirha owenziweyo iimvavanyo ezininzi zombini umfazi kunye izinto enikiweyo. Omnye yemiqathango esekwe kwi yebhanki ova engumkhenkce, kuba lithiwe pahaha. Oko kukuthi, abazali andazi malunga, ze vice versa. Iimfuno abafazi abaye bakhetha oocytes zabo vitrified:

1. Ubudala: Iminyaka 20-35.
2. Ukunqongophala izifo somatic kunye yemfuza.
3. Unkabi sho phenotype.
4. Ubukho abanikelayo ubuncinane omnye umntwana wabo.
5. Akavumanga wedwa.

Su vitrification

Phambi kokuba uqalise ngumkhenkce gametes ababhinqileyo, ebibanjwe ovulation ingqondo. Kuyimfuneko ukuba oocytes emininzi, kunokuba omnye. Emva zivuthwe yesiqhelo yenzeka ngexesha ovulation kunye follicle isivuno iqanda elinye kuphela. Ingqondo lwenziwa ngamayeza. Okulandelayo wenziwa a ultrasound zangasese. Ukuba ufumanisa ukuba ovulation yenzeke yaye oocytes kwi esizalweni ubuninzi ngokwaneleyo, le nkqubo-IVF kuyaqhutywa. ukuvuna Iseli yenziwa phantsi Ukubulawa jikelele. Ukuze kwenzakalani inaliti hit apho, apho oocytes, le nkqubo yonke lwenziwa phantsi kwesikhokelo ultrasound. Emva kokuthatha imathiriyeli ukuqalisa isigaba esilandelayo - ukugcinwa oocytes akuqolanga. Vitrification of oocytes kwenziwa kumjikelo zendalo ngenxa indlela ebandayo ngokukhawuleza. Olu phawu iphambili yale ndlela kulondolozwe.

Izinto eziluncedo ze vitrification of oocytes

Njengoko vitrikatsii inkqubo baqala kancinci indawo ezinye iindlela ukugcinwa ova Amadlozi, oko Kukhethwa nje kuphela koogqirha kodwa kwizigulane. Le ndlela iye iingenelo zilandelayo:

1. Ithuba ukuyi- ukukhulelwa. Ngenxa yokuba le oocytes kunye iimbumba emkhenkceni ixesha elide (kuxhomekeke kwisigqibo yomfazi).
2. Ukukwazi ngokutsha inkqubo-IVF angajongi izinto kumaxesha yesibini (okanye ngaphezulu).
3. Ukukwazi kumitha emva utyando kwi amadlala angasese, imitha kunye nechemotherapy.

Yintoni umahluko phakathi vitrification kunye cryopreservation amaqanda?

Njengoko yaziwa, iinkqubo kriokenservatsii kunye vitrification of oocytes iyafana kakhulu. Zombini iindlela zenzelwe 'amazinyo of oocytes kunye imibungu ukwenzela ukugcina nokusebenza kwabo. Nakuba kunjalo, kukho ezinye umahluko. Amaxesha amaninzi, ubuntwana kweqanda cryopreservation yonakele. Oku kungenxa yokuba le ndlela ibandakanya izinto neGauteng kade. Ngenxa yoko, ukuba kukho icytoplasm engamanzi ajikwa ice kwakheka. Ukuba kone udonga iseli. Indlela ogqwesileyo cryopreservation of oocytes yi vitrification. Ipesenti ndikufuna xa wenza asezantsi kakhulu zalo, njengoko Ukupholisa amaqanda kwenzeka phantse ngephanyazo. Yiloo nto ke umahluko eziphambili cryopreservation kuthathwa ubuchwepheshe obutsha ukugcina oocytes elide ukwimeko iphila. Yokulibazisa akhulelwe ukuba ungakwazi kuphela indlela vitrification ixesha elide. Cryopreservation ayinikeli ezinjalo iziphumo oluhlala luhleli.

Njani na IVF emva vitrification of oocytes?

Kweqanda vitrification wenziwa yonke indawo. Kokugcwaliswa of gametes Amadlozi ixesha elide waziphatha kuphela kwiilebhu ezizodwa kakhulu. Phantse zonke ikliniki nale inazo ibhanki oocytes zabaxhasi vitrified. Xa ibhinqa liye lagqiba ukuba ufuna ukukhawula umntwana, iiseli sele zinyibilikisiwe. Emva koko sebenzisa ubuchule ICSI luyenziwa isichumiso. Le mbumba ufakwa culturing (iintsuku 3-5) kwaye ingenisa zibe endometrium uzilungiselele. Iiseli eseleyo ukuba aqhamileyo kwakhona vitrified. Xa iinzame bamilisele inkqubo IVF iphindiwe.

Vitrification of oocytes: reviews oogqirha

Okwangoku, vitrification kunye nokugcinwa oocytes Amadlozi lwenziwa kuzo zonke izixeko ezinkulu zamachiza kuphuhliswa. Yakuba le ndlela iye ihlolwe kwaye iye yaba ngokwayo, oogqirha uthethe ngayo kakuhle, ukwishiba eziluncedo phezu kwezinye (ngaphambili) yokugcina iinkqubo amaqanda. Imali amisa oocytes yaso abafazi abaninzi. Inkqubo ixabisa malunga 12 amawaka engange. Le ntengo ukuba vitrification. Iindleko yokugcina iiseli frozen yi engange-1000 ngenyanga nganye.