Njani na isidoda cryopreservation

Kuba amadlozi ngesirinji yinto ethandwayo isidoda cryopreservation. Ngamadlozi yokugcina elide lula siqhuba IVF. Lo msebenzi kunye amadlozi umsebenzi ihlala ngenxa lobushushu eliphantsi - okupholisa kukuba nitrogen engamanzi. indlela yokugcina Innovative ikuvumela ukuba ukubuyisela amagciwane aphilayo, nokuba elishumi kwiminyaka kamva.

Kutheni kufuneka ebandayo ukusebenza

Isidoda cryopreservation kuyanconywa abantu njengoko kwesisombululo sengxaki ukungachumi. Le ndlela uba isisombululo ukuba abafazi abafuni ukuza wadibana umntu ogulayo. Yokuvala ntiyiso xa kukho ingozi yokosulela, kodwa akukho ngozi usana olungekazalwa.

Abantu siphambuka kwixesha elizayo xa ezisongela ekhoyo kwezempilo:

• iingxaki, izifo ezingapheliyo kwenkqubo enzala;
• Oncology eliphezulu;
• operation oluzayo emva kokonzakala;
• ukukhubazeka.

Londoloza izinto eziphilayo, yaye abantu abakulungeleyo ukwenza utyando ezayo, radiation okanye ichemotherapy. Kwakhona, abantu bakhetha iindlela zaselaborathri amazinyo umfazi i-inshorensi, ukuba baye emsebenzini kunye emngciphekweni omkhulu ebomini.

indlela vitrification nokugcina izinto eziphilayo:

• sperm;
• imibungu;
• iiseli ezibomvu;
• izibilini zangaphakathi;
• iqanda.

Isidoda Cryopreservation eMoscow ifumana abantu ngenxa ikhona. Ngakumbi nangakumbi iikliniki zinika iinkonzo ukulondoloza izinto eziphilayo. Abantu baqalisa ukusebenzisa le nkqubo njengomthombo wengeniso, ukuba luphile komzimba. Abanikelayo ziyafuneka kwabasetyhini izibini abaphethwe ukungachumi.

iinkqubo Basics

Khusela iiseli ngeempahla ezizodwa kwintshabalalo - cryoprotectant. ubungayi kuba nempumelelo kangaka ngaphandle ukusetyenziswa kweziyobisi ngumkhenkce.

Kodwa eziphilayo xa zisenziwa isidoda cryopreservation, impembelelo kunye izinto ezimbi;

• ukubunjwa ziikristale umkhenkce ngaphakathi iiseli kwiseli wamanzi;
• ngenxa yoko le nto yokuqala kukho emzimbeni.

Ukunqanda ukutshatyalaliswa iiseli uyahamba ke, bamana banikwa izisombululo cryoprotectant. izinto nokhuseleko zihlelwa njongo:

• gaze - kunciphisa crystallization bolwelo phakathi kwiiseli;
• nonpenetrating - iyavumelana kuphela Uhlobo lokuqala ukhuselo, ezijonge kukhuselwe iseli ukusuka babe zizinto ezimbi lwangaphandle.

Iqela lokuqala luquka: ethylene ethers, sulfoxide dimethyl, propylene ethers. izinto ezongezelelweyo: sucrose, ficoll. inkqubo defrosting Reverse sikhatshwa amanyathelo okukhutshwa iiseli abaphila kwamaqhina namazwe eSADC cryoprotectants.

Ukulungiselela nokugcina iinkqubo

Phambi kokuba isidoda cryopreservation kuliwa, wazithwala phandle uviwo olupheleleyo lwe imeko abanikelayo yomzimba. Uvavanyo ephambili analysis isidoda iba spermogram. Ikliniki enze uvavanyo trial yovalo izinto mntu. Kulindeleke ukuba ukuhlola ikhono imbewu lokuhlawulisa yokugcina ixesha elide.

Ekuthiwa-kriotest inceda oogqirha bahlole ukuba irisithi njengesiqinisekiso madlozi esempilweni. Iimvavanyo ezongezelelweyo yenzelwe ukukhusela umntwana ongekazalwa kwakunye nonina wayo ukususela ekusulelekeni:

• herpes simplex virus;
• Hepatitis B no C;
• usulelo lwe-HIV;
• negcushuwa.

Ngesicelo ngumthengi ingaqhuba ngokokuhamba zoviwo ezipheleleyo kubalizi. Ezi mvavanyo ziqhutywe imizuzu engama-20 emva kokuba inkqubo thawing. Ukuba zonke iitsheki ngamanyathelo HIV, isidoda zixubene nezisombululo ze cryoprotectants baze bafakwe kwindawo yokugcina okhethekileyo.

Indlela ukugcina biomaterial

Kwimigqomo apho sisebenzisa ulwelo amadlozi, kufuneka zigcinwe lobushushu -196 ° C. Azuze imiqathango efanelekileyo usebenzisa izinto ezithile - nitrogen engamanzi.

Human Sperm Cryopreservation lwenziwa kwiityhubhu ezinepolypropylene (iimpahla Dewar), apho biomaterial ize igcinwe. Le philisi efanayo kakhulu ibekwe kwisingxobo ne -nitrogen engamanzi.

lulondolozo lwexesha elide iminyaka emi-3, ngexesha ngemvumelwano kunye mali. It ukuba ukuhanjiswa leekhonteyina kwezinye izibhedlele kwingqiqo ngumthengi. Oku kuya kufuna isiqinisekiso esibhaliweyo umnqweno ukuthutha amadlozi zibambene. Le isigulane yakhupha iphepha ngexesha wokukhutshwa biomaterial.

Ithuba lokugcinwa ngokupheleleyo ejaculate, lingana ne ukuya kwiminyaka emithathu. Timeout akuqinisekisi ukugcinwa komgangatho izinto eziphilayo, ekubeni inkqubo yotshintsho lwangaphakathi weeseli iqala. nangoku Medicine akanawo amandla ngumkhenkce elingenammiselo, ukuze lowo wayingcwalisayo uthe ugqiba kwelokuba ukugcina isidoda kwisingxobo, okanye ukuphinda inkqubo preservation.

Ntoni ixabiso nkonzo ixhomekeke

Ukuxhaphaka iziphakamiso kwiiklinikhi zabucala kuphakamisa le ngxaki yokukhetha mbutho ngokunyaniseka, eqhuba inkqubo qualitative. Iiklinikhi Ungasebenzisa indlela zemveli yovalo - a slow sperm cryopreservation. Ixabiso le nkonzo yohlukile kancinci, kodwa kukho imingcipheko yomonakalo ejaculate. indlela ngakumbi ezixhaphakileyo vitrification - inkqubo ulondolozo superfast of biomaterial.

I-avareji yeendleko zeenkonzo ezichazwe bafike nakuma-10 400 engange. kwaye kuyahlukana nedlela zinto zilandelayo:

• Clinic udumo, inani ngeenkonzo ezenziwa kunye nohlolo HIV;
• cryopreservation indlela (ukuqanda kade okanye vitrification);
• umhla ukusayina isivumelwano - intlawulo epheleleyo yeendleko ixesha elide yehliswe kakhulu;
• iimeko zokugcina (ngokubanzi ichibi okanye kwisingxobo esahlukileyo).

Kuba yokugcina ngeembumba inyanga-1 kuya kufuneka ukuba uhlawule 1000 engange., Ke zokufunda yokugcina kwigumbi otedlnoe kuba xesha liya kuxabisa 4000 engange.

Imbali ivela indlela neGauteng

Ukuthembakala kwenkqubo eyavelayo emva kokuba ukuqaliswa ngempumelelo kwe-iiseli kunye nokuzalwa komntwana esempilweni eNetherlands. Iziganeko asusela kunyaka-1984, xa cryopreservation madlozi iye yavavanywa ngempumelelo. Technology iye yasinda kuzo ixesha lethu, kodwa kubekho inkqubela ebonakalayo inkqubo amazinyo.

amadlozi ngesirinji namhlanje kubangela iimvakalelo ezingalunganga kuluntu. Umbuzo usahleli:

• ukubhaliswa amalungelo asemthethweni komntwana, ukusekwa maternity kunye lukatata;
• agcinwe zokuziphatha icala ekumitheni indlela nemvelo;
• uluntu amanye amaqela zasempuma engekakulungeli ukuba indlela entsha yokuphila.

Kodwa phezu kwazo nje iingxaki, abantu baya usebenzisa cryopreservation njengendlela wokulondoloza impilo yosana elizayo.